Opacification of Middlebrook agar as an aid in distinguishing Nocardia farcinica within the Nocardia asteroides complex

Author:

Carson M1,Hellyar A1

Affiliation:

1. Department of Microbiology, Royal Melbourne Hospital, Australia.

Abstract

Among 58 aerobic actinomycetes isolated from different sources and geographical locations, none of 23 Nocardia asteroides isolates, at 18 N. farcinica isolates, 1 of 5 N. otitidiscaviarum isolates, and 1 of 4 Rhodococcus species isolates opacified Middlebrook 7H10 medium. Within the N. asteroides complex, this characteristic, together with growth at 45 degrees C and resistance to each of erythromycin, cefotaxime, and tobramycin, provides a simple means of distinguishing N. farcinica from N. asteroides.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference10 articles.

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4. Land G. M. McGinnis J. Staneck and A. Gaston. 1991. Aerobic pathogenic actinomycetales p. 340-359. In A. Balows W. J. Havsler Jr. K. L. Herrmann H. D. Isenberg and H. J. Shadomy (ed.) Manual of clinical microbiology 5th Ed. American Society for Microbiology Washington D.C.

5. Le Chevalier H. A. 1989. Nocardioform Actinomycetes p. 2348-2404. In S. T. Williams M. E. Sharpe and J. G. Holt (ed.) Bergey's manual of systematic bacteriology vol. 4 9th ed. The Williams & Wilkins Co. Baltimore.

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