Affiliation:
1. Helminth Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA
Abstract
ABSTRACT
Antigen-based immunoassays are currently needed for point-of-care quantification of
Loa loa
microfilariae (mf). Coupling transcriptomic approaches with bioinformatic analysis, we have identified 11 specific putative proteins (coding mRNAs) with potential utility as biomarkers of patent (mf
+
)
L. loa
infection. We successfully developed antigen capture immunoassays to quantify 2 (LOAG_14221 and LOAG_15846) of these proteins in individual plasma/serum samples. Of the 2 quantifiable circulating biomarkers, LOAG_14221 showed the highest degree of specificity, particularly with a monoclonal antibody-based immunoassay. Moreover, the levels of LOAG_14221 in
L. loa
mf
+
patients were positively correlated to the mf densities in the corresponding blood samples (
r
= 0.53 and
P
= 0.008 for polyclonal assay;
r
= 0.54 and
P
= 0.004 for monoclonal assay). Thus, LOAG_14221 is a very promising biomarker that will be exploited in a quantitative point-of-care immunoassay for determination of
L. loa
mf densities.
Publisher
American Society for Microbiology
Cited by
7 articles.
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