Rapid and Specific Method for Evaluating Streptomyces Competitive Dynamics in Complex Soil Communities

Author:

Schlatter Daniel C.1,Samac Deborah A.12,Tesfaye Mesfin3,Kinkel Linda L.1

Affiliation:

1. Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, Minnesota 55108

2. USDA-ARS-Plant Science Research Unit, St. Paul, Minnesota 55108

3. Department of Plant Biology, University of Minnesota, 250 Biological Sciences, 1445 Gortner Ave., St. Paul, Minnesota 55108

Abstract

ABSTRACT Quantifying target microbial populations in complex communities remains a barrier to studying species interactions in soil environments. Quantitative PCR (qPCR) assays were developed for quantifying pathogenic Streptomyces scabiei and antibiotic-producing Streptomyces lavendulae strains in complex soil communities. This assay will be useful for evaluating the competitive dynamics of streptomycetes in soil.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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