Affiliation:
1. Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
Abstract
The reverse genetics system has made it possible to modify the influenza virus genome. By this method, we were able to assess influenza virus as a vaccine vector for protecting BALB/c mice against otherwise lethal lymphocytic choriomeningitis virus (LCMV) infection. A single dose of influenza virus [A/WSN/33 (H1N1)] bearing a cytotoxic T-lymphocyte-specific epitope of the LCMV nucleoprotein (residues 116 to 127) in the neuraminidase stalk protected mice against LCMV challenge for at least 4 months. The immunity was mediated by cytotoxic T lymphocytes and was haplotype specific, indicating that the observed protective response was solely a consequence of prior priming with the H-2d LCMV nucleoprotein epitope expressed in the recombinant influenza virus. We also found that as many as 58 amino acids could be inserted into the neuraminidase stalk without loss of viral function. These findings demonstrate the potential of influenza virus as a vaccine vector, with the neuraminidase stalk as a repository for foreign epitopes.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference51 articles.
1. Virus specificity of cytotoxic T Iymphocytes generated during acute lymphocytic choriomeningitis virus infection: role of the H-2 region in determining cross-reactivity for different Iymphocytic choriomeningitis virus strains;Ahmed R.;J. Virol.,1984
2. Consequences of a single Ir-gene defect for the pathogenesis of Iymphocytic choriomeningitis;Allan J. E.;Immunogenetics,1985
3. Cellular events in the Iymph node and Iung of mice with influenza: consequences of depleting CD4+ T cells;Allan W.;J. Immunol.,1990
4. Window of vulnerability of vaccinia virus-infected cells to natural killer (NK) cell-mediated cytolysis correlates with enhanced NK cell triggering and is concomitant with a decrease in H-2 class I antigen expression;Brutkiewicz R. R.;Nat. Immun.,1992
5. Biology of cloned cytotoxic T Iymphocytes specific for Iymphocytic choriomeningitis virus: clearance of virus in vivo;Byrne J. A.;J. Virol.,1984
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