The Reliability of Metagenome-Assembled Genomes (MAGs) in Representing Natural Populations: Insights from Comparing MAGs against Isolate Genomes Derived from the Same Fecal Sample-Reference-Cited by-同舟云学术

The Reliability of Metagenome-Assembled Genomes (MAGs) in Representing Natural Populations: Insights from Comparing MAGs against Isolate Genomes Derived from the Same Fecal Sample

Published:2021-02-26 Issue:6 Volume:87 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Meziti Alexandra¹,Rodriguez-R Luis M.¹²³^ORCID,Hatt Janet K.¹,Peña-Gonzalez Angela⁴,Levy Karen⁵^ORCID,Konstantinidis Konstantinos T.¹⁴

Affiliation:

1. School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA

2. Department of Microbiology, University of Innsbruck, Innsbruck, Tyrol, Austria

3. Digital Science Center (DiSC), University of Innsbruck, Innsbruck, Tyrol, Austria

4. School of Biological Sciences, Georgia Institute of Technology, Atlanta, Georgia, USA

5. Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, Georgia, USA

Abstract

Metagenome assembly and the recovery of metagenome-assembled genomes (MAGs) have recently become common tasks for microbiome studies across environmental and clinical settings. However, the extent to which MAGs can capture the genes of the population they represent remains speculative.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

National Science Foundation

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02593-20

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