In Vivo Insertional Mutagenesis in Corynebacterium pseudotuberculosis : an Efficient Means To Identify DNA Sequences Encoding Exported Proteins-Reference-Cited by-同舟云学术

In Vivo Insertional Mutagenesis in Corynebacterium pseudotuberculosis : an Efficient Means To Identify DNA Sequences Encoding Exported Proteins

Published:2006-11 Issue:11 Volume:72 Page:7368-7372
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Dorella Fernanda A.¹,Estevam Estela M.¹,Pacheco Luis G. C.¹,Guimarães Cláudia T.²,Lana Ubiraci G. P.²,Gomes Eliane A.²,Barsante Michele M.³,Oliveira Sérgio C.³,Meyer Roberto⁴,Miyoshi Anderson¹,Azevedo Vasco¹

Affiliation:

1. Laboratório de Genética Celular e Molecular

2. Núcleo de Biologia Aplicada, Empresa Brasileira de Pesquisa Agropecuária, Sete Lagoas, Minas Gerais

3. Laboratório de Imunologia de Doenças Infecciosas, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte

4. Instituto de Ciências da Saúde, Vale do Canela, Salvador, Bahia, Brazil

Abstract

ABSTRACT The reporter transposon-based system TnFuZ was used to identify exported proteins of the animal pathogen Corynebacterium pseudotuberculosis . Thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity. This activity was from 21 C. pseudotuberculosis loci that code for fimbrial and transport subunits and for hypothetical and unknown-function proteins.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00294-06

Reference22 articles.

1. Billington, S. T., P. A. Esmay, J. G. Songer, and B. H. Jost. 2002. Identification and role in virulence of putative iron acquisition genes from Corynebacterium pseudotuberculosis. FEMS Microbiol. Lett.208:41-45.

2. Cerdeño-Tárraga, A. M., A. Efstratiou, L. G. Dover, M. T. Holden, M. Pallen, S. D. Bentley, G. S. Besra, C. Churcher, K. D. James, A. De Zoysa, T. Chillingworth, A. Cronin, L. Dowd, T. Feltwell, N. Hamlin, S. Holroyd, K. Jagels, S. Moule, M. A. Quail, E. Rabbinowitsch, K. M. Rutherford, N. R. Thomson, L. Unwin, S. Whitehead, B. G. Barrell, and J. Parkhill. 2003. The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129. Nucleic Acids Res.31:6516-6523.

3. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence

4. Dorella, F. A., E. M. Estevam, P. G. Cardoso, B. M. Savassi, S. C. Oliveira, V. Azevedo, and A. Miyoshi. 2006. An improved protocol for electrotransformation of Corynebacterium pseudotuberculosis. Vet. Microbiol.114:298-303.

5. Dorella, F. A., L. G. C. Pacheco, S. C. Oliveira, A. Miyoshi, and V. Azevedo. 2006. Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence. Vet. Res.37:201-218.

Cited by 17 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Corynebacterium pseudotuberculosis: Whole genome sequencing reveals unforeseen and relevant genetic diversity in this pathogen;PLOS ONE;2024-08-26

2. A multiomic approach to defining the essential genome of the globally important pathogen Corynebacterium diphtheriae;PLOS Genetics;2023-04-26

3. A journey through the Corynebacterium pseudotuberculosis proteome promotes insights into its functional genome;PeerJ;2021-12-23

4. The Transcriptional Regulatory Network of Corynebacterium pseudotuberculosis;Microorganisms;2021-02-17

5. Cell wall glycolipids from Corynebacterium pseudotuberculosis strains with different virulences differ in terms of composition and immune recognition;Brazilian Journal of Microbiology;2020-07-25