Cloning and Expression of the Listeria monocytogenes Scott A ptsH and ptsI Genes, Coding for HPr and Enzyme I, Respectively, of the Phosphotransferase System-Reference-Cited by-同舟云学术

Cloning and Expression of the Listeria monocytogenes Scott A ptsH and ptsI Genes, Coding for HPr and Enzyme I, Respectively, of the Phosphotransferase System

Published:1998-09 Issue:9 Volume:64 Page:3147-3152
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Christensen Douglas P.¹,Benson Andrew K.²,Hutkins Robert W.²

Affiliation:

1. School of Biological Sciences,1 and

2. Department of Food Science and Technology,2 University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0919

Abstract

ABSTRACT The phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) utilizes high-energy phosphate present in PEP to drive the uptake of several different carbohydrates in bacteria. In order to examine the role of the PTS in the physiology of Listeria monocytogenes , we identified the ptsH and ptsI genes encoding the HPr and enzyme I proteins, respectively, of the PTS. Nucleotide sequence analysis indicated that the predicted proteins are nearly 70% similar to HPr and enzyme I proteins from other organisms. Purified L. monocytogenes HPr overexpressed in Escherichia coli was also capable of complementing an HPr defect in heterologous extracts of Staphylococcus aureus ptsH mutants. Additional studies of the transcriptional organization and control indicated that the ptsH and ptsI genes are organized into a transcription unit that is under the control of a consensus-like promoter and that expression of these genes is mediated by glucose availability and pH or by by-products of glucose metabolism.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.64.9.3147-3152.1998

Reference26 articles.

1. Identification of a gene encoding an HPr-like protein in Aspergillus fumigatus.;Barker S.;J. Med. Vet. Mycol.,1991

2. The phosphoenolpyruvate-dependent phosphotransferase system of Staphylococcus aureus. I. Amino-acid sequence of the phosphocarrier protein HPr.;Beyreuther K.;Eur. J. Biochem.,1977

3. A gene encoding a superoxide dismutase of the facultative intracellular bacterium Listeria monocytogenes.;Brehm K.;Gene,1992

4. Construction of a new vector for the expression of foreign genes in Zymomonas mobilis.;Byun M. O.;J. Ind. Microbiol.,1986

5. Characterization of a new class of tetracycline-resistence gene tet(s) in Listeria monocytogenes BM4210.;Charpentier E.;Gene,1993

Cited by 13 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. DegU-mediated suppression of carbohydrate uptake in Listeria monocytogenes increases adaptation to oxidative stress;Applied and Environmental Microbiology;2023-10-31

2. Investigation of the Listeria monocytogenes Scott A Acid Tolerance Response and Associated Physiological and Phenotypic Features via Whole Proteome Analysis;Journal of Proteome Research;2012-03-15

3. Mutational analysis of glucose transport regulation and glucose‐mediated virulence gene repression inListeria monocytogenes;Molecular Microbiology;2011-05-25

4. Listeria monocytogenes PrsA2 Is Required for Virulence Factor Secretion and Bacterial Viability within the Host Cell Cytosol;Infection and Immunity;2010-11

5. Regulation of Mannose Phosphotransferase System Permease and Virulence Gene Expression in Listeria monocytogenes by the EII t Man Transporter;Applied and Environmental Microbiology;2009-11