Decrease in Cryptosporidium parvum Oocyst Infectivity In Vitro by Using the Membrane Filter Dissolution Method for Recovering Oocysts from Water Samples

Author:

Carreno Ramon A.1,Pokorny Nicholas J.1,Weir Susan C.1,Lee Hung1,Trevors Jack T.1

Affiliation:

1. Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1

Abstract

ABSTRACT Exposure of Cryptosporidium parvum oocysts to solutions used for cellulose acetate membrane (CAM) dissolution filtration reduced their infectivity in HCT-8 cells. Ethanol (95% [vol/vol] and 70% [vol/vol]) alone and short exposure times to acetone decreased infectivity. These findings contrast with similar experiments using excystation assays and infectivity in mice.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference26 articles.

1. Recovery of Cryptosporidium oocysts from water by a membrane filter dissolution method;Aldom J. E.;Lett. Appl. Microbiol.,1995

2. Aldom J. E. Chagla A. H. (1997) Membrane filter dissolution: a novel method for the recovery of Cryptosporidium oocysts from water. in 1997 International Symposium on Waterborne Cryptosporidium Proceedings. eds FrickerC. R.ClancyJ. L.RochelleP. A. (American Water Works Association Denver Colo) pp 87–89.

3. Blewett D. A. (1989) Disinfection and oocysts. in Cryptosporidiosis. Proceedings of the first international workshop. eds AngusK. W.BlewettD. A. (Moredun Research Institute The Animal Diseases Research Association Edinburgh United Kingdom) pp 107–115.

4. Viability of Cryptosporidium parvum oocysts: correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes

5. Complete development of Cryptosporidium in cell culture;Current W. L.;Science,1984

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