Affiliation:
1. Riken Institute, Saitama-ken, Japan.
Abstract
Two alkali-sensitive mutants of alkalophilic Bacillus sp. strain C-125 were obtained. Mutant 38154 showed defective regulation of internal pH. Plasmids pALK1 and pALK2, containing DNA fragments different from those of the parent strain, were able to recover alkalophily in mutants 18224 and 38154, respectively. DNA analysis suggested that the two fragments overlapped on the chromosomal DNA of strain C-125.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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