Shuttle mutagenesis of Neisseria gonorrhoeae: pilin null mutations lower DNA transformation competence-Reference-Cited by-同舟云学术

Shuttle mutagenesis of Neisseria gonorrhoeae: pilin null mutations lower DNA transformation competence

Published:1990-01 Issue:1 Volume:172 Page:40-46
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Seifert H S¹,Ajioka R S¹,Paruchuri D¹,Heffron F¹,So M¹

Affiliation:

1. Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.

Abstract

The method of shuttle mutagenesis has been extended to Neisseria gonorrhoeae. We have constructed a defective mini-Tn3 derivative that encodes chloramphenicol resistance in both N. gonorrhoeae and Escherichia coli and selected for mutations in the chloramphenicol resistance gene that express higher levels of antibiotic resistance in N. gonorrhoeae. Isogenic N. gonorrhoeae strains that differ only in pilin expression were constructed and used to test the effect of pilin null mutations on DNA transformation competence.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.172.1.40-46.1990

Reference34 articles.

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