groE genes affect SOS repair in Escherichia coli

Author:

Liu S K1,Tessman I1

Affiliation:

1. Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.

Abstract

Repair of UV-irradiated bacteriophage in Escherichia coli by Weigle reactivation requires functional recA+ and umuD+C+ genes. When the cells were UV irradiated, the groE heat shock gene products, GroES and GroEL, were needed for at least 50% of the Weigle reactivation of the single-stranded DNA phage S13. Because of repression of the umuDC and recA genes, Weigle reactivation is normally blocked by the lexA3(Ind-) mutation (which creates a noncleavable LexA protein), but it was restored by a combination of a high-copy-number umuD+C+ plasmid and a UV dose that increases groE expression. Maximal reactivation was achieved by elevated amounts of the Umu proteins, which was accomplished in part by UV-induced expression of the groE genes. By increasing the number of copies of the umuD+C+ genes, up to 50% of the normal amount of reactivation of S13 was achieved in an unirradiated recA+ host.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference26 articles.

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2. Induction of prophage A without amplification of recA protein;Baluch J.;Mol. Gen. Genet.,1980

3. D'Ari R. and 0. Huisman. 1982. DNA replication and indirect induction of the SOS response in Escherichia coli. Biochimie 6138 NOTES

4. groE mutants of Escherichia coli are defective in umuDC-dependent UV mutagenesis;Donnelly C. E.;J. Bacteriol.,1989

5. Dulbecco R. 1955. Photoreactivation p. 455-486. In A. Hollaender (ed.) Radiation biology: ultraviolet and related radiations vol. 2. McGraw-Hill Book Co. New York.

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