Morphogenesis of Poliovirus II. Demonstration of a New Intermediate, the Proviron-Reference-Cited by-同舟云学术

Morphogenesis of Poliovirus II. Demonstration of a New Intermediate, the Proviron

Published:1973-11 Issue:5 Volume:12 Page:1122-1130
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Fernandez-Tomas Carlos B.¹,Baltimore David¹

Affiliation:

1. Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

Abstract

Poliovirus-infected cells contain a previously unrecognized particle which appears to be an intermediate in virion synthesis and therefore has been named proviron. It sediments at about 125 S , contains the three procapsid proteins, VP-0, VP-1, and VP-3, and has 35 S viral RNA. It is disrupted both by sodium dodecyl sulfate and EDTA but the RNA resists digestion by ribonuclease. Pulsechase experiments and studies employing the virus-specific inhibitor, guanidine, all indicate that the proviron is formed by combination of newly made RNA with the procapsid. Cleavage of VP-0 to form VP-2 and VP-4 follows formation of the provirion and would be the final step in poliovirus morphogenesis.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.12.5.1122-1130.1973

Reference23 articles.

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3. Baltimore D. 1969. The replication of picornaviruses p. 101-176. In H. B. Levy (ed.) The biochemistry of viruses. Marcel Dekker New York.

4. Baltimore D. 1971. Polio is not dead p. 1-12. In M. Pollard (ed.) From molecules to man perspectives in virology VII. Academic Press Inc. New York.

5. An intermediate in the synthesis of poliovirus RNA;Baltimore D.;Proc. Nat. Acad. Sci. U.S.A.,1966

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