Biophysical and Dynamic Characterization of Fine-Tuned Binding of the Human Respiratory Syncytial Virus M2-1 Core Domain to Long RNAs-Reference-Cited by-同舟云学术

Biophysical and Dynamic Characterization of Fine-Tuned Binding of the Human Respiratory Syncytial Virus M2-1 Core Domain to Long RNAs

Published:2020-11-09 Issue:23 Volume:94 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Caruso Icaro P.¹²³⁴,Guimarães Giovana C.¹,Machado Vitor B.¹,Fossey Marcelo A.¹²,Willbold Dieter⁵⁶,Almeida Fabio C. L.³⁴^ORCID,Souza Fátima P.¹²

Affiliation:

1. Multiuser Center for Biomolecular Innovation (CMIB), IBILCE/UNESP, São José do Rio Preto, SP, Brazil

2. Department of Physics, IBILCE/UNESP, São José do Rio Preto, SP, Brazil

3. National Center for Nuclear Magnetic Resonance of Macromolecules, Institute of Medical Biochemistry Leopoldo de Meis (IBqM), UFRJ, Rio de Janeiro, RJ, Brazil

4. National Center for Structural Biology and Bioimaging (CENABIO), UFRJ, Rio de Janeiro, RJ, Brazil

5. Institute of Biological Information Processing, Structural Biochemistry and JuStruct (IBI-7), Forchungszentrum Jülich, Jülich, Germany

6. Institut für Physikalische Biologie, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany

Abstract

The main outcome is the molecular description of the fine-tuned binding of the cdM2-1/RNA complex and the provision of evidence that the domain alone has unfolding activity for long RNAs. This binding mode is essential in the understanding of the function in the full-length protein. Human respiratory syncytial virus (hRSV), an orthopneumovirus, stands out for the unique role of its M2-1 protein as a transcriptional antitermination factor able to increase RNA polymerase processivity.

Funder

MCTI | Conselho Nacional de Desenvolvimento Científico e Tecnológico

Fundação de Amparo à Pesquisa do Estado de São Paulo

Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.01505-20

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