Detection and Quantitation of Methanogens by Enzyme-Linked Immunosorbent Assay

Author:

Archer David B.1

Affiliation:

1. Agricultural and Food Research Council Food Research Institute, Colney Lane, Norwich NR4 7UA, United Kingdom

Abstract

Antisera to two methanogenic bacteria, Methanosarcina barkeri and a Methanobacterium sp., were raised in rabbits and used to develop an enzyme-linked immunosorbent assay (ELISA) method. ELISA was shown to be a sensitive technique, detecting as little as 4 ng of methanogen protein. The specificities of the antisera toward other methanogens were evaluated, and it was found that the antisera recognized species of the same genus as the immunizing species, but gave very little cross-reaction with methanogens of different genera. ELISA was used to estimate the growth of methanogens in pure culture. In natural environments and in anaerobic digesters methanogens exist as part of a mixed bacterial community, so the possibility of using ELISA to quantitate methanogens in mixed cultures was examined. The two antisera gave very little reaction in ELISA when non-methanogenic bacteria were used as antigens and ELISA was used to quantitate methanogens in an acetate enrichment culture. I conclude that the ELISA is a useful method for quantitating methanogens in defined mixed cultures, but has limited applicability to more complex systems.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference21 articles.

1. Hydrogen-using bacteria in a methanogenic acetate enrichment culture;Archer D. B.;J. Appl. Bacteriol.,1983

2. Serological relatedness of spiroplasmas estimated by enzyme-linked immunosorbent assay and crossed immunoelectrophoresis;Archer D. B.;J. Gen. Microbiol.,1980

3. A novel ultrastructural feature of a gas-vacuolated Methanosarcina;Archer D. B.;FEMS Microbiol. Lett.,1983

4. Methanogens: reevaluation of a unique biological group;Balch W. E.;Microbiol. Rev.,1979

5. Recent advances in anaerobic digestion technology;Callander I. J.;Process Biochem.,1983

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