Affiliation:
1. Marine Biology Research Division and Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, California 92093-0202
Abstract
ABSTRACT
A multicopper oxidase gene,
cumA,
required for Mn(II) oxidation was recently identified in
Pseudomonas putida
strain GB-1. In the present study, degenerate primers based on the putative copper-binding regions of the
cumA
gene product were used to PCR amplify
cumA
gene sequences from a variety of
Pseudomonas
strains, including both Mn(II)-oxidizing and non-Mn(II)-oxidizing strains. The presence of highly conserved
cumA
gene sequences in several apparently non-Mn(II)-oxidizing
Pseudomonas
strains suggests that this gene may not be expressed, may not be sufficient alone to confer the ability to oxidize Mn(II), or may have an alternative function in these organisms. Phylogenetic analysis of both CumA and 16S rRNA sequences revealed similar topologies between the respective trees, including the presence of several distinct phylogenetic clusters. Overall, our results indicate that both the
cumA
gene and the capacity to oxidize Mn(II) occur in phylogenetically diverse
Pseudomonas
strains.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
111 articles.
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