Metachromatic Agar-Diffusion Methods for Detecting Staphylococcal Nuclease Activity

Author:

Lachica R. V. F.123,Genigeorgis C.123,Hoeprich P. D.123

Affiliation:

1. Department of Microbiology, Weber State College, Ogden, Utah

2. Section of Infectious and Immunologic Diseases, Department of Internal Medicine, School of Medicine, University of California, Davis, California 95616

3. Department of Epidemiology and Preventive Medicine, School of Veterinary Medicine, University of California, Davis, California 95616

Abstract

Based on the metachromatic property of Toluidine Blue O, three, convenient agar-diffusion methods have been developed that enable detection of the nuclease of Staphylococcus aureus at concentrations as low as 0.005 μg/ml in agar and broth cultures. The interactions of agar and deoxyribonucleic acid with Toluidine Blue O are discussed.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference6 articles.

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