Ribotyping: a tool for taxonomy and identification of the Nocardia asteroides complex species-Reference-Cited by-同舟云学术

Ribotyping: a tool for taxonomy and identification of the Nocardia asteroides complex species

Published:1996-05 Issue:5 Volume:34 Page:1079-1082
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Laurent F¹,Carlotti A¹,Boiron P¹,Villard J¹,Freney J¹

Affiliation:

1. Department de Recherche en Bacteriologie (E.A. 1655), Faculté de Médecine A. Carrel, Lyon, France.

Abstract

Ribotyping has been evaluated as a taxonomic tool for studying bacteria belonging to the Nocardia asteroides complex. The probe used was obtained by PCR from DNA extracted from the type strain Nocardia asteroides ATCC 19247, a sequence that codes partly for 16S rRNA. Interpretation of hybridization after EcoRI restriction of total DNA of 21 strains of the N. asteroides complex allowed for the identification of only four different ribotypes: two related to N. asteroides sensu stricto, one related to N. farcinica and one related to N. nova. These results confirm the classification established by Tsukamura in 1982 (M. Tsukamura, Microbiol. Immunol. 26:1101-1119, 1982) as well as the heterogeneity of N. asteroides sensu stricto species. Ribotyping may also provide a major identification technique for the species belonging to the N. asteroides complex.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.34.5.1079-1082.1996

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