Affiliation:
1. Institut National de la Santé et de la Recherche Médicale, Unité 431, Faculté de Médecine, Nîmes, France.
Abstract
Thirty-four Brucella reference or field strains representing all the species and biovars were studied by repetitive element sequence-based PCR, a PCR using primers complementary to two enterobacterial short repetitive sequences: repetitive extragenic palindromic and enterobacterial repetitive intergenic consensus sequences. All the stains showed a positive amplification, suggesting that the Brucella genome contains such sequences. Repetitive extragenic palindromic PCR was less discriminating than enterobacterial repetitive intergenic consensus PCR in terms of distinguishing strains, but a combination of the two methods was able to distinguish all the isolates, except for some strains belonging to biovars 3 and 9 of Brucella abortus. Repetitive element sequence-based PCR appears to be a simple and useful method for the study of brucellosis epidemiology.
Publisher
American Society for Microbiology
Reference19 articles.
1. DNA polymorphism in strains of the genus Brucella;Allardet-Servent A.;J. Bacteriol.,1988
2. Use of low-frequency cleavage restriction endonucleases in epidemiological investigations of nosocomial bacterial infections;Allardet-Servent A.;J. Clin. Microbiol.,1989
3. Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR;Bricker B. J.;J. Clin. Microbiol.,1994
4. Use of repetitive (repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus) sequences and the polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria;de Bruijn F. J.;Appl. Environ. Microbiol.,1992
5. Amplification fragment length polymorphism in Brucella strains by use of polymerase chain reaction with arbitrary primers;Fekete A.;J. Bacteriol.,1992
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