Novel use of polymerase chain reaction to amplify cellular DNA adjacent to an integrated provirus-Reference-Cited by-同舟云学术

Novel use of polymerase chain reaction to amplify cellular DNA adjacent to an integrated provirus

Published:1989-05 Issue:5 Volume:63 Page:1924-1928
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Silver J¹,Keerikatte V¹

Affiliation:

1. Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.

Abstract

We describe a modification of the polymerase chain reaction technique which allows amplification of cellular DNA adjacent to an integrated provirus given sequence information for the provirus only. The modified technique should be generally useful for studies of insertional mutagenesis and other situations in which one wishes to isolate DNA adjacent to a region of known sequence.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.63.5.1924-1928.1989

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