In Vivo 23 Na Nuclear Magnetic Resonance Study of Maintenance of a Sodium Gradient in the Ruminal Bacterium Fibrobacter succinogenes S85-Reference-Cited by-同舟云学术

In Vivo 23 Na Nuclear Magnetic Resonance Study of Maintenance of a Sodium Gradient in the Ruminal Bacterium Fibrobacter succinogenes S85

Published:2001-09 Issue:9 Volume:67 Page:4390-4392
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Schwaab Véronique¹²,Matheron Christelle¹,Delort Anne-Marie¹,Gaudet Geneviève²,Forano Evelyne²

Affiliation:

1. Laboratoire de Synthèse, Electrosynthèse et Etude de Systèmes àIntérêt Biologique, UMR 6504, Université Blaise Pascal—CNRS, 63177 Aubière cedex,1 and

2. Unité de Microbiologie, INRA, Centre de Recherches de Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle,2 France

Abstract

ABSTRACT Sodium gradients (ΔpNa) were measured in resting cells of Fibrobacter succinogenes by in vivo 23 Na nuclear magnetic resonance using Tm(DOTP) 5− [thulium(III) 1,4,7,10-tetraazacyclododecane- N ′, N ′′, N ′′′-tetramethylenephosphonate] as the shift reagent. This bacterium was able to maintain a ΔpNa of −55 to −40 mV for extracellular sodium concentrations ranging from 30 to 200 mM. Depletion of Na + ions during the washing steps led to irreversible damage (modification of glucose metabolism and inability to maintain a sodium gradient).

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.67.9.4390-4392.2001

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