Phenolic acridine orange fluorescent stain for mycobacteria-Reference-Cited by-同舟云学术

Phenolic acridine orange fluorescent stain for mycobacteria

Published:1995-10 Issue:10 Volume:33 Page:2763-2764
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Smithwick R W¹,Bigbie M R¹,Ferguson R B¹,Karlix M A¹,Wallis C K¹

Affiliation:

1. Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.

Abstract

A new fluorescence acid-fast staining method with acridine orange as the specific stain is presented. Only two reagents are required: the acridine orange-specific stain and a destaining-counterstaining reagent. Compared with auramine fluorescence acid-fast staining, there was less nonspecific staining of non-acid-fast debris which fluoresced a pale green contrasting color to provide a background in which to search for the red-to-orange fluorescing acid-fast bacilli. The results of the study indicate that the acridine orange method is superior to the auramine method in detecting acid-fast bacilli in specimen smears.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.33.10.2763-2764.1995

Reference10 articles.

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5. Detection of small numbers of mycobacteria in sections by fluorescence microscopy;Gray D. F.;Am. Rev. Tuberc.,1953

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