Affiliation:
1. Departments of Molecular Microbiology and Immunology1 and
2. Medicine and Neurology,2 Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205
Abstract
ABSTRACT
Alphaviruses are arthropod-borne viruses with wide species ranges and diverse tissue tropisms. The cell surface receptors which allow infection of so many different species and cell types are still incompletely characterized. We show here that the widely expressed glycosaminoglycan heparan sulfate can participate in the binding of Sindbis virus to cells. Enzymatic removal of heparan sulfate or the use of heparan sulfate-deficient cells led to a large reduction in virus binding. Sindbis virus bound to immobilized heparin, and this interaction was blocked by neutralizing antibodies against the viral E2 glycoprotein. Further experiments showed that a high degree of sulfation was critical for the ability of heparin to bind Sindbis virus. However, Sindbis virus was still able to infect and replicate on cells which were completely deficient in heparan sulfate, indicating that additional receptors must be involved. Cell surface binding of another alphavirus, Ross River virus, was found to be independent of heparan sulfate.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference72 articles.
1. Studies of viral virulence. I. Growth and histopathology of virulent and attenuated strains of Venezuelan encephalitis virus in hamsters;Austin F. J.;Am. J. Pathol.,1971
2. Sulphated and undersulphated heparan sulphate proteoglycans in a Chinese hamster ovary cell mutant defective in N-sulphotransferase;Bame K. J.;Biochem. J.,1994
3. Separation of Sindbis virus plaque-type variants by calcium phosphate chromatography;Bose H. R.;Arch. Gesamte Virusforsch.,1970
4. Some factors affecting plaque size of western equine encephalomyelitis virus;Brown L. N.;Am. J. Vet. Res.,1964
5. Byrnes A. P. and D. E. Griffin. Unpublished data.
Cited by
253 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献