First Case of Type E Wound Botulism Diagnosed Using Real-Time PCR

Author:

Artin Ingrid12,Björkman Per3,Cronqvist Jonas3,Rådström Peter2,Holst Elisabet1

Affiliation:

1. Division of Medical Microbiology, Department of Laboratory Medicine, Lund University, SE-223 62 Lund

2. Applied Microbiology, Lund Institute of Technology, Lund University, SE-221 00 Lund

3. Division of Infectious Diseases, Department of Clinical Sciences, Malmö University Hospital, Lund University, Malmö, Sweden

Abstract

ABSTRACT Wound botulism is a growing problem among injecting drug users. The condition is often difficult to diagnose, with laboratory confirmation in only 50% of the cases. Here we present a real-time PCR-based method for the diagnosis of wound botulism caused by Clostridium botulinum . The assay includes an internal amplification control which is amplified simultaneously with the genes encoding neurotoxin types A, B, and E. This method was used to detect the first case of wound botulism in an injecting drug user in Sweden. In addition, to the best of our knowledge, this is the first reported case of wound botulism caused by C. botulinum type E.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference36 articles.

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2. Akbulut, D., K. A. Grant, and J. McLauchlin. 2004. Development and application of real-time PCR assays to detect fragments of the Clostridium botulinum types A, B, and E neurotoxin genes for investigation of human foodborne and infant botulism. Foodborne Pathog. Dis.1:247-257.

3. Alpers, K., U. van Treeck, and C. Frank. 2005. Outbreak of wound botulism in injecting drug users in Germany, October-December 2005. Euro Surveill.10:E051215.4.

4. Alsallami, A. A., and R. Kotlowski. 2001. Selection of primers for specific detection of Clostridium botulinum types B and E neurotoxin genes using PCR method. Int. J. Food Microbiol.69:247-253.

5. Brett, M. M., G. Hallas, and O. Mpamugo. 2004. Wound botulism in the UK and Ireland. J. Med. Microbiol.53:555-561.

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