First Case of Type E Wound Botulism Diagnosed Using Real-Time PCR-Reference-Cited by-同舟云学术

First Case of Type E Wound Botulism Diagnosed Using Real-Time PCR

Published:2007-11 Issue:11 Volume:45 Page:3589-3594
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Artin Ingrid¹²,Björkman Per³,Cronqvist Jonas³,Rådström Peter²,Holst Elisabet¹

Affiliation:

1. Division of Medical Microbiology, Department of Laboratory Medicine, Lund University, SE-223 62 Lund

2. Applied Microbiology, Lund Institute of Technology, Lund University, SE-221 00 Lund

3. Division of Infectious Diseases, Department of Clinical Sciences, Malmö University Hospital, Lund University, Malmö, Sweden

Abstract

ABSTRACT Wound botulism is a growing problem among injecting drug users. The condition is often difficult to diagnose, with laboratory confirmation in only 50% of the cases. Here we present a real-time PCR-based method for the diagnosis of wound botulism caused by Clostridium botulinum . The assay includes an internal amplification control which is amplified simultaneously with the genes encoding neurotoxin types A, B, and E. This method was used to detect the first case of wound botulism in an injecting drug user in Sweden. In addition, to the best of our knowledge, this is the first reported case of wound botulism caused by C. botulinum type E.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01192-07

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