Affiliation:
1. Institute of Genetics, University of Pavia, Pavia, Italy
Abstract
The relationships among deoxyribonucleic acid (DNA) uptake, transformation, and autoradiographic labeling were investigated. It is shown that: (i) autoradiography is a good method for measuring the total fraction of competent cells able to incorporate transforming DNA; in our best experiment that fraction was 11.5%. (ii) Computation of the fraction of competent cells in a culture of
Bacillus subtilis
, by comparing the frequencies of single and double transformants for two unlinked markers, gives values which are somewhat different from those obtained by auto-radiography. (iii) On the average, a competent cell of
B. subtilis
irreversibly takes up from 0.9 × 10
−10
to 1.4 × 10
−10
μg of DNA; this amount represents about 1/55 to 1/35 of the bacterial chromosome weighing 5 × 10
−9
μg, and corresponds to three to six DNA molecules having a molecular weight of 1.6 × 10
7
.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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