Identification and Functional Analysis of Escherichia coli Cysteine Desulfhydrases-Reference-Cited by-同舟云学术

Identification and Functional Analysis of Escherichia coli Cysteine Desulfhydrases

Published:2005-07 Issue:7 Volume:71 Page:4149-4152
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Awano Naoki¹,Wada Masaru¹,Mori Hirotada²,Nakamori Shigeru¹,Takagi Hiroshi¹

Affiliation:

1. Department of Bioscience, Fukui Prefectural University, 4-1-1 Kenjojima, Matsuoka-cho, Fukui 910-1195

2. Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Ikoma 630-0101, Japan

Abstract

ABSTRACT In Escherichia coli , three additional proteins having l -cysteine desulfhydrase activity were identified as O -acetylserine sulfhydrylase-A, O -acetylserine sulfhydrylase-B, and MalY protein, in addition to tryptophanase and cystathionine β-lyase, which have been reported previously. The gene disruption for each protein was significantly effective for overproduction of l -cysteine and l -cystine. Growth phenotype and transcriptional analyses suggest that tryptophanase contributes primarily to l -cysteine degradation.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.71.7.4149-4152.2005

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