Elucidating the Key Member of a Linuron-Mineralizing Bacterial Community by PCR and Reverse Transcription-PCR Denaturing Gradient Gel Electrophoresis 16S rRNA Gene Fingerprinting and Cultivation-Reference-Cited by-同舟云学术

Elucidating the Key Member of a Linuron-Mineralizing Bacterial Community by PCR and Reverse Transcription-PCR Denaturing Gradient Gel Electrophoresis 16S rRNA Gene Fingerprinting and Cultivation

Published:2005-07 Issue:7 Volume:71 Page:4144-4148
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Sørensen Sebastian R.¹,Rasmussen Jim¹²,Jacobsen Carsten S.¹³,Jacobsen Ole S.¹,Juhler René K.¹,Aamand Jens¹

Affiliation:

1. Department of Geochemistry, Geological Survey of Denmark and Greenland (GEUS), Øster Voldgade 10, DK-1350 Copenhagen K, Denmark

2. Department of Agricultural Sciences, The Royal Veterinary and Agricultural University (KVL), Højbakkegårds Allé 30, DK-2630 Tåstrup, Denmark

3. Department of Natural Sciences and Department of Ecology, KVL, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark

Abstract

ABSTRACT A bacterial community from Danish agricultural soil was enriched with linuron [ N -(3,4-dichlorophenyl)- N ′-methoxy- N ′-methylurea] as the sole carbon and nitrogen source. The community mineralized [ ring -U- 14 C]linuron completely to 14 CO 2 and 14 C-biomass. Denaturing gradient gel electrophoresis analysis and cultivation revealed that a Variovorax sp. was responsible for the mineralization activity.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.71.7.4144-4148.2005

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