A Cluster of Genes Involved in Polysaccharide Biosynthesis from Enterococcus faecalis OG1RF-Reference-Cited by-同舟云学术

A Cluster of Genes Involved in Polysaccharide Biosynthesis from Enterococcus faecalis OG1RF

Published:1998-09 Issue:9 Volume:66 Page:4313-4323
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Xu Yi¹,Murray Barbara E.²³⁴,Weinstock George M.¹²³⁴

Affiliation:

1. Department of Biochemistry and Molecular Biology,1

2. Department of Microbiology and Molecular Genetics,2

3. Division of Infectious Diseases, Department of Medicine,3and

4. Center for the Study of Emerging and Re-Emerging Pathogens,4 University of Texas Medical School, Houston, Texas 77030

Abstract

ABSTRACT Our previous work identified a cosmid clone containing a 43-kb insert from Enterococcus faecalis OG1RF that produced a nonprotein antigen in Escherichia coli . In the present work, we studied this clone in detail. Periodate treatment of lysates of the clone confirmed that the antigen was carbohydrate in nature. Analysis of DNA sequences and transposon insertion mutants suggested that the insert contained a multicistronic gene cluster. Database comparison showed that the cluster contained genes similar to genes involved in the biosynthesis of dTDP-rhamnose, glycosyltransferases, and ABC transporters involved in the export of sugar polymers from both gram-positive and gram-negative organisms. Insertions in several genes within the cluster abolished the immunoreactivity of the clone. This is the first report on a gene cluster of E. faecalis involved in the biosynthesis of an antigenic polysaccharide.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.66.9.4313-4323.1998

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