Abstract
To localize the structural gene for outer membrane protein e, mutants resistant to the protein e-specific phage TC45 were isolated and characterized. Three classes of TC45-resistant strains were found: (i) mutants in phoB, a regulatory gene for protein e, (ii) mutants with an altered lipopolysaccharide, and (iii) mutants unaltered in the regulation of the pho regulon and producing an apparently normal lipopolysaccharide. Mutations in the latter class of mutants are probably altered in the structural gene for protein e and are cotransducible with proA,B at min 6 on the chromosomal map. As class (iii) includes mutants with an electrophoretically altered protein e, in both an nmpA and an nmpB background, we conclude that the structural gene for protein e, designated as phoE, is localized at min 6 on the chromosomal map, the gene order being phoE proA argF.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference29 articles.
1. Linkage map of Escherichia coli K-12, edition 6;Bachmann B. J.;Microbiol. Rev.,1980
2. Mutants of Escherichia coli "cryptic;Beacham L. R.;J. Bacteriol.,1977
3. Characterization of lipopolysaccharide from Escherichia coli K-12 mutants;Boman H. G.;J. Bacteriol.,1975
4. Inactivation of bacteriophages by protein E, a new major membrane protein isolated from an Ewcherichia coli mutant;Chai T.;J. Bacteriol.,1979
5. New major outer membrane protein found in an Escherichia coli tolF mutant resistant to bacteriophage TuIb;Foulds J.;J. Bacteriol.,1978
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