Isolation and Characterization of a Xylanase from Bacillus subtilis-Reference-Cited by-同舟云学术

Isolation and Characterization of a Xylanase from Bacillus subtilis

Published:1983-08 Issue:2 Volume:46 Page:511-514
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Bernier Roger¹,Desrochers Michel¹,Jurasek Lubomir¹,Paice Michael G.¹

Affiliation:

1. Pulp and Paper Research Institute of Canada, Pointe Claire, Quebec H9R 3J9, Canada

Abstract

Partial characterization of an extracellular xylanase isolated by chromatography from Bacillus subtilis gave a molecular weight of 32,000 and optimum pH and temperature of 5.0 and 50�C, respectively. K m and V max values, determined with a soluble larchwood xylan, were 0.16% and 7.0 � 10 3 μmol min −1 mg −1 of enzyme respectively. The amino acid composition showed more basic amino acid residues than in a previously characterized xylanase from a white-rot fungus.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.46.2.511-514.1983

Reference21 articles.

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