Affiliation:
1. Bacteriology Laboratory, Central Leather Research Institute, Madras-600 020, India
Abstract
Tannase isolated from
Penicillium chrysogenum
was purified 24-fold with 18.5% recovery after ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex G-200 gel filtration. Optimum enzyme activity was recorded at pH 5.0 to 6.0 and at 30 to 40�C. The enzyme was stable up to 30�C and within the pH range of 4.0 to 6.5. The
K
m
value was found to be 0.48 � 10
−4
M when tannic acid was used as the substrate. Metal salts at 20 mM inhibited the enzyme to different levels.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference13 articles.
1. Studies on fungal tannase. II. Physico-chemical properties of tannase of A. flavus;Adachi O.;Agric. Biol. Chem.,1968
2. Purification of some properties of yeast tannase;Aoki K.;Agric. Biol. Chem.,1976
3. Chemical composition and molecular weight of yeast tannase;Aoki K.;Agric. Biol. Chem.,1976
4. Disc electrophoresis. II. Method and application to human serum proteins;Davis B. J.;Ann. N. Y. Acad. Sci.,1964
5. Gallotannins. I. Introduction and fractionation of tannase;Haslam E.;J. Chem. Soc.,1961
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