Affiliation:
1. Division of Environmental and Nutritional Sciences, School of Public Health, University of California, Los Angeles, California 90024
Abstract
Growth of
Methanosarcina
sp. strain 227 and
Methanosarcina mazei
on H
2
-CO
2
and mixtures of H
2
-CO
2
and acetate or methanol was examined. The growth yield of strain 227 on H
2
-CO
2
in complex medium was 8.4 mg/mmol of methane produced. Growth in defined medium was characteristically slower, and cell yields were proportionately lower. Labeling studies confirmed that CO
2
was rapidly reduced to CH
4
in the presence of H
2
, and little acetate was used for methanogenesis until H
2
was exhausted. This resulted in a biphasic pattern of growth similar to that reported for strain 227 grown on methanol-acetate mixtures. Biphasic growth was not observed in cultures on mixtures of H
2
-CO
2
and methanol, and less methanol oxidation occurred in the presence of H
2
. In
M. mazei
the aceticlastic reaction was also inhibited by the added H
2
, but since the cultures did not immediately metabolize H
2
, the duration of the inhibition was much longer.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
81 articles.
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