Affiliation:
1. Department of Plant Pathology and Microbiology1 and
2. Department of Chemistry, Johns Hopkins University, Baltimore, Maryland2
3. Department of Biology,3 Texas A&M University, College Station, Texas, and
Abstract
ABSTRACT
Sterigmatocystin (ST) and aflatoxin B
1
(AFB
1
) are two polyketide-derived
Aspergillus
mycotoxins synthesized by functionally identical sets of enzymes. ST, the compound produced by
Aspergillus nidulans
, is a late intermediate in the AFB
1
pathway of
A. parasiticus
and
A. flavus
. Previous biochemical studies predicted that five oxygenase steps are required for the formation of ST. A 60-kb ST gene cluster in
A. nidulans
contains five genes,
stcB
,
stcF
,
stcL
,
stcS
, and
stcW
, encoding putative monooxygenase activities. Prior research showed that
stcL
and
stcS
mutants accumulated versicolorins B and A, respectively. We now show that strains disrupted at
stcF
, encoding a P-450 monooxygenase similar to
A. parasiticus avnA
, accumulate averantin. Disruption of either StcB (a putative P-450 monooxygenase) or StcW (a putative flavin-requiring monooxygenase) led to the accumulation of averufin as determined by radiolabeled feeding and extraction studies.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
45 articles.
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