Rapid Method for Coextraction of DNA and RNA from Natural Environments for Analysis of Ribosomal DNA- and rRNA-Based Microbial Community Composition-Reference-Cited by-同舟云学术

Rapid Method for Coextraction of DNA and RNA from Natural Environments for Analysis of Ribosomal DNA- and rRNA-Based Microbial Community Composition

Published:2000-12 Issue:12 Volume:66 Page:5488-5491
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Griffiths Robert I.¹²,Whiteley Andrew S.¹,O'Donnell Anthony G.²,Bailey Mark J.¹

Affiliation:

1. Molecular Microbial Ecology Laboratory, Institute of Virology and Environmental Microbiology, CEH-Oxford, Oxford OX1 3SR,1 and

2. Department of Agricultural and Environmental Science, The University of Newcastle Upon Tyne, Newcastle Upon Tyne NE1 7RU,2 United Kingdom

Abstract

ABSTRACT A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription-PCR amplification from a single extraction. Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.66.12.5488-5491.2000

Reference15 articles.

1. Creating a ribonuclease-free environment;Blumberg D. D.;Methods Enzymol.,1987

2. Extraction of ribosomal RNA and genomic DNA from soil for studying the diversity of the indigenous bacterial community;Duarte G. F.;J. Microb. Methods,1998

3. Direct ribosome isolation from soil to extract bacterial rRNA for community analysis

4. Phylogeny of the Main Bacterial 16S rRNA Sequences in Drentse A Grassland Soils (The Netherlands)

5. Quantification of Bias Related to the Extraction of DNA Directly from Soils

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