Affiliation:
1. Molecular Diagnostics Unit, Cork Institute of Technology, Bishopstown, Cork, Ireland
Abstract
ABSTRACT
Two hundred and twenty-six
Salmonella enterica
serotype Typhimurium isolates were examined for the presence of integron-associated gene cassettes. All but two of the non-DT104 isolates, together with DT104 isolates, contained gene cassettes. Amplicons of 1.5 kbp each were found in two non-DT104 isolates, encoding a
dhfrI
gene (trimethoprim resistance) linked to an
aadA
gene (streptomycin and spectinomycin resistance), by site-specific recombination. DT104 isolates of resistance (R) type ACSSuT possessed the recently described 1.0- and 1.2-kbp gene cassettes. Macrorestriction analysis with
Xba
I and DNA probing mapped
ant
(
3"
)-
1a
,
bla
PSE-1
, and
dhfrI
genes to large multiresistant gene clusters in a DT170a isolate and a DT193 isolate. In contrast, all DT104 isolates (R-type ACSSuT) possessed a conserved 10-kbp
Xba1
DNA fragment. Our study highlights the occurrence of integrons (and antimicrobial resistance determinants) among serotype Typhimurium isolates other than DT104. Larger and previously unrecognized multiresistance gene clusters were identified in these isolates by DNA probing.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
45 articles.
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