Specific Detection of Leuconostoc mesenteroides subsp. mesenteroides with DNA Primers Identified by Randomly Amplified Polymorphic DNA Analysis-Reference-Cited by-同舟云学术

Specific Detection of Leuconostoc mesenteroides subsp. mesenteroides with DNA Primers Identified by Randomly Amplified Polymorphic DNA Analysis

Published:2000-01 Issue:1 Volume:66 Page:422-424
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Moschetti Giancarlo¹,Blaiotta Giuseppe¹,Villani Francesco¹,Coppola Salvatore¹

Affiliation:

1. Dipartimento di Scienza degli Alimenti, Sezione di Microbiologia Agraria, Alimentare ed Ambientale e di Igiene, Università degli Studi di Napoli “Federico II,” I 80055 Portici, Italy

Abstract

ABSTRACT Randomly amplified polymorphic DNA analysis using primer 239 (5′ CTGAAGCGGA 3′) was performed to characterize Leuconostoc sp. strains. All the strains of Leuconostoc mesenteroides subsp. mesenteroides (with the exception of two strains), two strains formerly identified as L. gelidum , and one strain of Leuconostoc showed a common band at about 1.1 kb. This DNA fragment was cloned and sequenced in order to verify its suitability for identifying L. mesenteroides subsp. mesenteroides strains.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.66.1.422-424.2000

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