Affiliation:
1. Bacterial Pathogenesis Research Group, Department of Microbiology, School of Biomedical Sciences, Monash University, Victoria, 3800, Australia
Abstract
ABSTRACT
We have previously shown that a
Pasteurella multocida cexA
mutant (PBA875) was impaired in capsule export and highly attenuated in virulence for mice (J. D. Boyce and B. Adler, Infect. Immun. 68:3463–3468, 2000). In this study we show that immunization with high, but not low, doses of PBA875 can confer significant protection against wild-type challenge. We have also constructed a genetically defined acapsular
P. multocida
strain (AL18) by inactivation of
bcbH
, a gene predicted to be involved in polysaccharide biosynthesis. AL18 failed to produce immunoreactive polysaccharide as determined by immunofluorescence and Western immunoblot. Immunization of mice with live AL18 conferred significant protection against wild-type challenge, while immunization with similar doses of either killed wild-type or killed AL18 failed to confer protection.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference30 articles.
1. Immunity and vaccine development in Pasteurella multocida infections;Adler B.;J. Biotechnol.,1996
2. Bain
R. V. S.
De Alwis
M. C. L.
Carter
G. R.
Gupta
B. K.
Haemorrhagic septicaemia
FAO Animal Production and Health Paper 33.
1982
11
33
Food and Agriculture Organization of the United Nations
Rome Italy
3. The molecular epidemiology of four outbreaks of porcine pasteurellosis;Blackall P. J.;Vet. Microbiol.,2000
4. The capsule is a virulence determinant in the pathogenesis of Pasteurella multocida M1404 (B:2);Boyce J. D.;Infect. Immun.,2000
5. Genetic organisation of the capsule biosynthetic locus of Pasteurella multocida M1404 (B:2);Boyce J. D.;Vet. Microbiol.,2000
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