Molecular Characterization of the PhoP-PhoQ Two-Component System in Escherichia coli K-12: Identification of Extracellular Mg 2+ -Responsive Promoters

Author:

Kato Akinori1,Tanabe Hiroyuki1,Utsumi Ryutaro1

Affiliation:

1. Department of Agricultural Chemistry, Kinki University, 3327-204, Nakamachi, Nara 631-8505, Japan

Abstract

ABSTRACT We identified Mg 2+ -responsive promoters of the phoPQ , mgtA , and mgrB genes of Escherichia coli K-12 by S1 nuclease analysis. Expression of these genes was induced by magnesium limitation and depended on PhoP and PhoQ. The transcription start sites were also determined, which allowed us to find a (T/G)GTTTA direct repeat in their corresponding promoter regions.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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