Affiliation:
1. Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801
Abstract
ABSTRACT
We have previously reported the isolation of a group of anaerobically regulated,
fnr
-dependent
lac
fusions in
Salmonella typhimurium
and have grouped these
oxd
genes into classes based on map position. In order to identify these genes, we have replaced the original Mud-
lac
fusion in a member of each
oxd
class with the much smaller Mud-
cam
element, cloned the fusion, and determined DNA sequence sufficient to define the
oxd
gene. Several of the fusions correspond to previously known genes from
S. typhimurium
or
Escherichia coli
:
oxd-4 = cbiA
and
oxd-11 = cbiK
,
oxd-5 = hybB
,
oxd-7 = dcuB
,
oxd-8 = moaB
,
oxd-12 = dmsA
, and
oxd-14 = napB
(
aeg-46.5
). Two other fusions correspond to previously unknown loci:
oxd-2
encodes an acetate/propionate kinase, and
oxd-6
encodes a putative ABC transporter present in
S. typhimurium
but not in
E. coli
.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
18 articles.
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