Affiliation:
1. Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709
Abstract
ABSTRACT
Upon infecting populations of susceptible host cells, T-even bacteriophages maximize their yield by switching from lysis at about 25 to 35 min at 37°C after infection by a single phage particle to long-delayed lysis (lysis inhibition) under conditions of sequential infection occurring when free phages outnumber host cells. The timing of lysis depends upon gene
t
and upon one or more rapid-lysis (
r
) genes whose inactivation prevents lysis inhibition.
t
encodes a holin that mediates the movement of the T4 endolysin though the inner cell membrane to its target, the cell wall. The rI protein has been proposed to sense superinfection. Of the five reasonably well characterized
r
genes, only two,
rI
and
rV
, are clearly obligatory for lysis inhibition. We show here that
rV
mutations are alleles of
t
that probably render the t protein unable to respond to the lysis inhibition signal. The
tr
alleles cluster in the 5′ third of
t
and produce a strong r phenotype, whereas conditional-lethal
t
alleles produce the classical t phenotype (inability to lyse) and other
t
alleles produce additional, still poorly understood phenotypes.
tr
mutations are dominant to
t
+
, a result that suggests specific ways to probe T4 holin function.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference40 articles.
1. Lysis of lysis-inhibited bacteriophage T4-infected cells
2. Abedon
S. T.
Lysis and the interaction between free phages and infected cells
Molecular biology of bacteriophage T4.
Karam
J. D.
Drake
J. W.
Kreuzer
K. N.
Mosig
G.
Hall
D. H.
Eiserling
F. A.
Black
L. W.
Spicer
E. K.
Kutter
E.
Carlson
K.
Miller
E. S.
1994
397
405
American Society for Microbiology
Washington D.C
3. Abedon S. T. Bacteriophage T4 resistance to lysis-inhibition collapse. Genet. Res. in press.
4. Two beginnings for a single purpose: the dual-start holins in the regulation of phage lysis;Bläsi U.;Mol. Microbiol.,1996
5. S gene expression and the timing of lysis by bacteriophage lambda
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