Affiliation:
1. Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa 761-0795, Japan
Abstract
ABSTRACT
We have cloned a gene,
pdcA
, from the genomic library of
Myxococcus xanthus
with an oligonucleotide probe representing conserved regions of penicillin-resistant
dd
-carboxypeptidases. The amino- and carboxy-terminal halves of the predicted
pdcA
gene product showed significant sequence similarity to
N
-acetylmuramoyl-
l
-alanine amidase and penicillin-resistant
dd
-carboxypeptidase, respectively. The
pdcA
gene was expressed in
Escherichia coli
, and the characteristics of the gene product were similar to those of
dd
-carboxypeptidase (VanY) of vancomycin-resistant enterococci. No apparent changes in cell growth, sporulation, or germination were observed in
pdcA
deletion mutants.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
10 articles.
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