Affiliation:
1. Faculty of Pharmaceutical Sciences, Chiba University, Inage-ku, Chiba 263-8522, Japan
Abstract
ABSTRACT
A K
+
/H
+
antiporter regulates cytoplasmic pH in
Enterococcus hirae
growing at alkaline pH. Mutants defective in this antiport activity were alkaline pH sensitive. One mutant, Pop1, lacked both K
+
/methylamine exchange at pH 9.5 and concomitant acidification of cytoplasmic pH. Pop1 grew well at pHs below 8 but did not at pHs above 9, conditions under which cytoplasmic pH was not fully acidified.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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