Affiliation:
1. Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
Abstract
ABSTRACT
High-level expression of the major pilus subunit (PapA) of uropathogenic strains of
Escherichia coli
results in part from the unusually long lifetime of the mRNA that encodes this protein. Here we report that the longevity of
papA
mRNA derives in large measure from the protection afforded by its 5′ untranslated region. This
papA
RNA segment can prolong the lifetime of an otherwise short-lived mRNA to which it is fused. In vivo alkylation studies indicate that, in its natural milieu, the
papA
message begins with a stem-loop structure. This stem-loop is important for the stabilizing effect of the
papA
5′ untranslated region, as evidenced by the significant acceleration in
papA
mRNA decay that results from its removal.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
45 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献