Affiliation:
1. Centro Nacional de Biotecnologı́a, Consejo Superior de Investigaciones Cientı́ficas, Campus Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain
Abstract
ABSTRACT
The sequence of a 4.8-kbp DNA fragment adjacent to the right-hand end of the actinorhodin biosynthetic (
act
) cluster downstream of
actVB-orf6
from
Streptomyces coelicolor
A3(2) reveals six complete open reading frames, named
orf7
to
orf12
. The deduced amino acid sequences from
orf7
,
orf10
, and
orf11
show significant similarities with the following products in the databases: a putative protein from the
S. coelicolor
SCP3 plasmid, LysR-type transcriptional regulators, and proteins belonging to the family of short-chain dehydrogenases/reductases, respectively. The deduced product of
orf8
reveals low similarities with several methyltransferases from different sources, while
orf9
and
orf12
products show no similarities with other known proteins. Disruptions of
orf10
and
orf11
genes in
S. coelicolor
appear to have no significant effect on the production of actinorhodin. Nevertheless, disruption or deletion of
orf10
in
Streptomyces lividans
causes actinorhodin overproduction. The introduction of extra copies of
orf10
and
orf11
genes in an
S. coelicolor actIII
mutant restores the ability to produce actinorhodin. Transcriptional analysis and DNA footprinting indicate that Orf10 represses its own transcription and regulates
orf11
transcription, expression of which might require the presence of an unknown inducer. No DNA target for Orf10 protein was found within the
act
cluster.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
16 articles.
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