Persistence of Rhizobium japonicum on the Soybean Seed Coat Under Controlled Temperature and Humidity

Author:

Davidson F.1,Reuszer H. W.2

Affiliation:

1. Research Seeds, Inc., St. Joseph, Missouri 64501

2. Department of Agronomy, Purdue University, Lafayette, Indiana 47907

Abstract

When Rhizobium japonicum strain 61A68 was added to surface-sterilized soybean ( Glycine max ) seed along with 12 different coating materials, a definite effect of temperature upon survival was observed both with and without coating materials. At a storage temperature of 15°C and 50 ± 5% relative humidity, from 0.9 to 14.1% of the original inoculum survived for 3 weeks. At 22.5°C, from 0.5 to 7.2% of the original inoculum survived. At 30°C, from 0.1 to 1.6% of the original inoculum survived. The data indicated that extremely large numbers of R. japonicum would have to be added to the seed to have numbers adequate for nodulation survive for 3 weeks of storage at ordinary temperatures.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference10 articles.

1. Studies on seed pelleting as an aid to legume seed. I. Coating materials, adhesives and methods of inoculation;Brockwell J.;Aust. J. Agric. Res.,1962

2. Brockwell J. Application of legume seed inoculants. In R. W. F. Hardy and A. H. Gibson (ed.) A treatise on dinitrogen fixation sect. IV. John Wiley & Sons Inc. New York.

3. Burton J. C. 1976. Problems in obtaining adequate inoculation of soybeans. In. L. D. Hill (ed.) World soybean research. Interstate Printers & Publishers Inc. Danville Ill.

4. Burton J. C. 1976. Methods of inoculating seeds and their effect on survival of rhizobia p. 175-189. In P. S. Nutman (ed.) Symbiotic nitrogen fixation in plants vol. 7. Cambridge University Press Cambridge.

5. Comparative efficiency of liquid and peat-base inoculants on fieldgrown soybeans (Glycine max);Burton J. C.;Agron. J.,1965

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