In vitro susceptibilities of suspected periodontopathic anaerobes as determined by membrane transfer assay

Author:

Caufield P W1,Allen D N1,Childers N K1

Affiliation:

1. Institute of Dental Research, University of Alabama, Birmingham 35294.

Abstract

Attempts to devise an antimicrobial approach to combating dentomicrobial infections such as periodontal diseases continue to be hampered by the lack of a relevant in vitro method for determining the susceptibility of suspected periodontopathogens to topically applied antimicrobial agents. Proposed here is a novel in vitro method called the membrane transfer technique, which acknowledges those aspects unique to localized pathogenic infections, particularly those associated with anaerobic bacteria. Bacterial lawns representing six suspected periodontopathic bacteria were prepared on membranes and then placed in contact with different concentrations of antimicrobial agents for 5 min. After incubation for 12 to 24 h, MBCs were determined with the aid of a tetrazolium chloride indicator. Four antimicrobial agents (chlorhexidine, iodine, stannous fluoride, and sodium fluoride) were used to test the applicability of the proposed in vitro method. MBCs were derived for each agent except sodium fluoride against all or most of the six bacterial strains tested. The proposed method may also be useful for examining the bactericidal action of topically applied antimicrobial agents against nonoral infections.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference24 articles.

1. Antibiotic susceptibility of anaerobic bacteria from the human oral cavity;Baker P. J.;J. Dent. Res.,1985

2. Suppression of Streptococcus mutans in the mouths of humans by a dental prophylaxis and topically-applied iodine;Caufield P. W.;J. Dent. Res.,1979

3. Caufield P. W. and J. M. Navia. 1980. Antimicrobial agents for management of dental caries p. 386-418. In L. Menaker (ed.) The biological basis of dental caries. Harper and Row Hagerstown Md.

4. Studies on the "carry-over;Chabbert Y. A.;J. Clin. Pathol.,1965

5. Studies on the predominant cultivable microbiota of dental plaque;Gibbons R. J.;Arch. Oral Biol.,1964

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