Novel Use of Tryptose Sulfite Cycloserine Egg Yolk Agar for Isolation of Clostridium perfringens during an Outbreak of Necrotizing Enterocolitis in a Neonatal Unit

Author:

Kotsanas Despina1,Carson Jolene A.2,Awad Milena M.3,Lyras Dena3,Rood Julian I.3,Jenkin Grant A.13,Stuart Rhonda L.14,Korman Tony M.15

Affiliation:

1. Department of Infectious Diseases, Southern Health, Monash Medical Centre, Clayton, Melbourne, Victoria, Australia 3168

2. Microbiology Department, Southern Cross Pathology Australia, Monash Medical Centre, Clayton, Melbourne, Victoria, Australia 3168

3. Department of Microbiology, Monash University, Clayton, Victoria, Australia 3800

4. Department of Infection Control, Southern Health, Monash Medical Centre, Clayton, Melbourne, Victoria, Australia 3168

5. Department of Medicine, Monash University, Wellington Road, Clayton, Melbourne, Victoria, Australia 3168

Abstract

ABSTRACT Clostridium perfringens has been associated with necrotizing enterocolitis (NEC), which is a serious disease of neonates. Our study describes the novel use of selective tryptose sulfite cycloserine with egg yolk agar (TSC-EYA) during a nursery outbreak. This medium provides a rapid, sensitive, and accurate presumptive identification of C. perfringens .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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