Identification of the Hepatitis C Virus RNA Replication Complex in Huh-7 Cells Harboring Subgenomic Replicons-Reference-Cited by-同舟云学术

Identification of the Hepatitis C Virus RNA Replication Complex in Huh-7 Cells Harboring Subgenomic Replicons

Published:2003-05 Issue:9 Volume:77 Page:5487-5492
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Gosert Rainer¹²,Egger Denise²,Lohmann Volker³,Bartenschlager Ralf³,Blum Hubert E.¹,Bienz Kurt²,Moradpour Darius¹

Affiliation:

1. Department of Medicine II, University of Freiburg, D-79106 Freiburg

2. Institute for Medical Microbiology, University of Basel, CH-4003 Basel, Switzerland

3. Department of Molecular Virology, University of Heidelberg, D-69120 Heidelberg, Germany

Abstract

ABSTRACT Formation of a membrane-associated replication complex, composed of viral proteins, replicating RNA, and altered cellular membranes, is a characteristic feature of plus-strand RNA viruses. Here, we demonstrate the presence of a specific membrane alteration, designated the membranous web, that contains hepatitis C virus (HCV) nonstructural proteins, as well as viral plus-strand RNA, in Huh-7 cells harboring autonomously replicating subgenomic HCV RNAs. Metabolic labeling with 5-bromouridine 5′-triphosphate in the presence of actinomycin D revealed that the membranous web is the site of viral RNA synthesis and therefore represents the replication complex of HCV.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.77.9.5487-5492.2003

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