Method for Testing Degree of Infectivity of Rhizobium meliloti Strains

Author:

Olivares Jos�1,Casades�s Josep1,Bedmar Eulogio J.1

Affiliation:

1. Departamento de Microbiolog�a, Estaci�n Experimental del Zaid�n del C.S.I.C., Granada, Spain

Abstract

The infectiveness of different strains of Rhizobium meliloti was tested with a technique that uses the addition of tetracycline to the root medium. To stop the infection, the antibiotic was added some time after the inoculation of Medicago sativa plants. A coefficient of infectivity for each strain was calculated according to the number of nodules that appeared with and without the addition of the antibiotic. This method seems useful in infectivity studies and is simpler and easier to perform than the test of competence between strains.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference14 articles.

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