Production of Glutaconic Acid in a Recombinant Escherichia coli Strain-Reference-Cited by-同舟云学术

Production of Glutaconic Acid in a Recombinant Escherichia coli Strain

Published:2011-01 Issue:1 Volume:77 Page:320-322
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Djurdjevic Ivana¹,Zelder Oskar²,Buckel Wolfgang¹³

Affiliation:

1. Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps-Universität, 35032 Marburg, Germany

2. BASF SE, Fermentation Products Research, GVF/D-A30, 67056 Ludwigshafen, Germany

3. Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Str. 10, 35043 Marburg, Germany

Abstract

ABSTRACT The assembly of six genes that encode enzymes from glutamate-fermenting bacteria converted Escherichia coli into a glutaconate producer when grown anaerobically on a complex medium. The new anaerobic pathway starts with 2-oxoglutarate from general metabolism and proceeds via ( R )-2-hydroxyglutarate, ( R )-2-hydroxyglutaryl-coenzyme A (CoA), and ( E )-glutaconyl-CoA to yield 2.7 ± 0.2 mM ( E )-glutaconate in the medium.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02049-10

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