Use of a Locked-Nucleic-Acid Oligomer in the Clamped-Probe Assay for Detection of a Minority Pfcrt K76T Mutant Population of Plasmodium falciparum-Reference-Cited by-同舟云学术

Use of a Locked-Nucleic-Acid Oligomer in the Clamped-Probe Assay for Detection of a Minority Pfcrt K76T Mutant Population of Plasmodium falciparum

Published:2005-07 Issue:7 Volume:43 Page:3304-3308
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Senescau Alice¹,Berry Antoine¹,Benoit-Vical Françoise¹²,Landt Olfert³,Fabre Richard¹,Lelièvre Joël¹²,Cassaing Sophie¹,Magnaval Jean-François¹

Affiliation:

1. Department of Parasitology, Rangueil University Hospital, 31059 Toulouse 9, France

2. Laboratoire de Chimie de Coordination du CNRS, 205 route de Narbonne, 31077 Toulouse 4, France

3. Tib Molbiol, Tempelhofer weg 11-12, D-10829 Berlin, Germany

Abstract

ABSTRACT Given the emergence of drug resistance and the high rate of polyclonal microorganism infections, the availability of a fast and sensitive test to detect minority mutant populations would be an improvement in the diagnosis of infectious diseases. A clamped-probe real-time PCR assay to diagnose the Plasmodium falciparum K76T mutation in clone populations was developed, using a wild-type-specific locked-nucleic-acid-containing oligomer to suppress wild-type PCR amplification and to enhance melting analysis with a mutation-specific detection probe.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.7.3304-3308.2005

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